Systems for applied gene control in Saccharomyces cerevisiae |
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Authors: | Douglas Maya María José Quintero María de la Cruz Muñoz-Centeno Sebastián Chávez |
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Affiliation: | (1) Departamento de Genética, Universidad de Sevilla, Avda. Reina Mercedes 6, 41012 Seville, Spain |
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Abstract: | Saccharomyces cerevisiae is frequently used in biotechnology, including fermentative processes in food production, heterologous protein production and high throughput developments for biomedicine. Accurate expression of selected genes is essential for all these areas. Systems that can be regulated are particularly useful because they allow controlling the timing and levels of gene expression. We examine here new expression systems that have been described, including improvements of classical ones and new strategies of artificial gene control that have been applied in functional genomics. |
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Keywords: | Basal expression Gene regulation Heterologous protein production Inducible promoter Saccharomyces cerevisiae |
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