Quantitation of rice glutelin,an acid/alkali/urea soluble protein,by enzyme linked immunosorbent assay |
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| Authors: | Y. R. Mawal M. R. Mawal M. N. Sainani P. K. Ranjekar |
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| Affiliation: | (1) Biochemical Sciences Division, National Chemical Laboratory, 411008 Pune, India |
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| Abstract: | Summary A sensitive and rapid ELISA for quantitation of rice glutelin was developed. This method employed binding of rice glutelin to Immulon I microtitre plate and quantitating it with commercial goat anti-rabbit IgG conjugate by conventional ELISA. Using this procedure, glutelin as low as 5ng could be detected. |
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