Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli |
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Authors: | Andreas Tauch,Oliver Kirchner,Lutz Wehmeier,Jö rn Kalinowski,Alfred Pü hler |
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Affiliation: | Department of Genetics, University of Bielefeld, P.O. Box 100131, 33501 Bielefeld, Germany |
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Abstract: | Abstract Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5αMCR was used instead of E. coli DH5α. We used E. coli strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system. |
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Keywords: | Corynebacterium glutamicum Restriction Modification Methylcytosine Electroporation Mcr system Mcr system |
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