Changes in the Content of Modified Nucleotides in Wheat rRNA during Greening |
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Authors: | I Parádi E Páldi S Rudnóy Z Bratek G Kovács I Rácz D Lásztity |
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Institution: | 1. Department of Plant Physiology, E?tv?s Loránd University, Pázmány Péter sétány 1/c, H-1117, Budapest, Hungary 3. Agricultural Research Institute of the Hungarian Academy of Sciences, POB 19, H-2462, Martonvásár, Hungary 2. Department of Microbiology, E?tv?s Loránd University, Pázmány Péter sétány 1/c, H-117, Budapest, Hungary
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Abstract: | The modified nucleotide content of the ribosomal RNAs in wheat is greatly influenced by light. The rRNAs of etiolated seedlings
contain far fewer modified derivatives. The modified nucleotide composition characteristic of green plants develops gradually
as a result of irradiation. In the course of the experiments changes in the state of modification of 5.8S and 18S rRNAs were
examined during the greening of etiolated wheat seedlings. Three types of minor nucleotides, O2′-methyladenosine, O2′-methylguanosine and pseudouridine were found in the 5.8S rRNA of green wheat leaves, none of which was detected in etiolated
wheat. The minor nucleotides appeared in the 5.8S rRNA only after 48 h irradiation. The sequences of 5.8S rDNA, TTS1, ITS2
and 18S rDNA were also determined and the presence of the hyper-modified nucleotide 1-methyl-3-(α-amino-α-carboxypropyl)-pseudouridine
was detected in green wheat 18S rRNA. This minor component was not demonstrable in etiolated wheat 18S rRNA, but appeared
after irradiation for 48 h.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | 5 8S rRNA 18S rRNA ITS1 and ITS2 hyper-modified pseudouridine |
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