首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Glycomimicry: display of fucosylation on the lipo-oligosaccharide of recombinant Escherichia coli K12
Authors:Elif Yavuz  Carola Maffioli  Karin Ilg  Markus Aebi  Bernard Priem
Institution:(1) CERMAV-CNRS, Grenoble, France;(2) Institute of Microbiology, ETH, Zurich, Switzerland;
Abstract:We recently described the design of Escherichia coli K12 and Salmonella enterica sv Typhimurium to display the gangliomannoside 3 (GM3) antigen on the cell surface 1]. We report here the fucosylation of modified lipooligosaccharide in a recombinant E.coli strain with a truncated lipid A core due to deletion of the core glycosyltransferases genes waaO and waaB. This truncated structure was used as a scaffold to assemble the Lewis Y motif by consequent action of the heterologously expressed β-1,4 galactosyltransferase LgtE (Neisseria gonorrheae), the β-1,3 N-acetylglucosaminyltransferase LgtA and the β-1,3 galactosyltransferase LgtB from Neisseria meningitidis, as well as the α-1,2 and α-1,3 fucosyltransferases FutC and FutA from Helicobacter pylori. We show the display of the Lewis Y structure by immunological and chemical analysis.
Keywords:
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号