Glycomimicry: display of fucosylation on the lipo-oligosaccharide of recombinant Escherichia coli K12 |
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Authors: | Elif Yavuz Carola Maffioli Karin Ilg Markus Aebi Bernard Priem |
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Institution: | (1) CERMAV-CNRS, Grenoble, France;(2) Institute of Microbiology, ETH, Zurich, Switzerland; |
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Abstract: | We recently described the design of Escherichia coli K12 and Salmonella enterica sv Typhimurium to display the gangliomannoside 3 (GM3) antigen on the cell surface 1]. We report here the fucosylation of modified lipooligosaccharide in a recombinant E.coli strain with a truncated lipid A core due to deletion of the core glycosyltransferases genes waaO and waaB. This truncated structure was used as a scaffold to assemble the Lewis Y motif by consequent action of the heterologously
expressed β-1,4 galactosyltransferase LgtE (Neisseria gonorrheae), the β-1,3 N-acetylglucosaminyltransferase LgtA and the β-1,3 galactosyltransferase LgtB from Neisseria meningitidis, as well as the α-1,2 and α-1,3 fucosyltransferases FutC and FutA from Helicobacter pylori. We show the display of the Lewis Y structure by immunological and chemical analysis. |
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