圆形碘泡虫免疫原性的研究

间接红细胞血凝试验结果表明，自然感染圆形泡虫的鲫鱼血清中存在循环抗体，并且感染强度与抗体水平不相关。以圆形碘泡虫孢子的可溶性蛋白为抗原，制备多抗。ＥＬＩＳＡ和ＩＦＡＴ试验表明，不同发育时期的圆形碘泡虫存在共同抗原，并且粘孢子虫具有属特异性抗原。圆形碘泡虫的抗原成分主要集中在早体后部的一特异位点及四周的早壁上，两个极囊无抗原成分；而 营养体的抗原成分存在于整个虫体。关碘泡虫与兔抗圆形碘泡虫抗体的结合

THE IMMUNOGENICITY OF MYXOBOLUS ROTUNDUS NEMECZEK, 1911

An indirect haemagglutination test was empolyed to detect circulating antibodies in the sera of Carassius auratus Linnaeus infected naturally with Wyxobolus rotundus Nemeczek. The circulating antibodies were detected in naturally infected C.auratus. But statistical correlation between infection intensities and antibody levels was found. Polyantibodies were prepared by injecting New Zealand white rabbit with soluble antigen of M rotundus. It was suggested that same antigens among different life stages of M .rotundus, and the genus-specific antigens be presented by using enzyme-linked immunoassay (ELISA). By using the indirect fluorescent antibody technique (IFAn, fluorescence was found densely concentrated in the rear point and periphery of spores of M. rotundus. However, the two polar capsules were obviously unstained in the fluorescent background. The anti-M. rotundus antiserum could react with M. guanqiaoensis Wu et Wang by IFAT, fluorescence was observed in sporoplasm and the anterior end of the sppre wall. At the same time, polar filaments were fainily fluorescent. Trophozoites of the two parasites could react with anti-M.rotundus antiserum. Fluorescence was scattered on the whole parasites.