Determination of rat pineal gland melatonin content by a radioimmunoassay. |
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| Authors: | R J Wurzburger K Kawashima R L Miller S Spector |
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| Institution: | 1. Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA;2. Jichi Medical School, Department of Pharmacology, Tochigi-Ken 329-04 Japan;3. Howard University Medical School, Freemans Hospital, Department of Medicine, Washington, D.C., 20001, USA |
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| Abstract: | Melatonin content in individual rat pineal glands was measured by radioimmunoassay (RIA). The RIA used can very reliably detect as little as 50 pg of melatonin. The various precursors, analogues, and the metabolite of melatonin (6-hydroxymelatonin) which were tested for cross-reactivity were not recognized by the antibody. The effects on melatonin levels in rat pineal glands following the administration of L-tryptophan, 5-hydroxy-L-tryptophan, serotonin, N-acetylserotonin, melatonin and pargyline are also presented. |
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