Regulatory elements in the promoter of the vitelline membrane gene VM32E of Drosophila melanogaster direct gene expression in distinct domains of the follicular epithelium |
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Authors: | V Cavaliere S Spanò D Andrenacci L Cortesi and G Gargiulo |
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Institution: | (1) Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Via Selmi 3, 40126 Bologna, Italy, IT |
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Abstract: | The Drosophila vitelline membrane protein gene VM32E is expressed according to a precise temporal and spatial program in the follicle cells. Results from germ line transformation
experiments using different fragments of the −465/−39 VM32E region fused to the hsp/lacZ reporter gene revealed that the region −348/−39 is sufficient to confer the wild-type expression pattern. Within this segment,
distinct cis-regulatory elements control VM32E expression in ventral and dorsal follicle cells. The region between −135/−113 is essential for expression of the VM32E gene in the ventral columnar follicle cells. Expression in the dorsal domain requires the two regions −348/−254 and −118/−39.
Furthermore, the region −253/−119 appears to contain a negative element that represses gene activity in anterior centripetal
cells. We suggest that the expression of the VM32E gene throughout the follicular epithelium is controlled by specific cis-regulatory elements acting in distinct spatial domains and following a precise developmental program.
Received: 22 October 1996 / Accepted: 14 November 1996 |
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Keywords: | Oogenesis Vitelline membrane genes Follicle cell functions Drosophila melanogaster |
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