Abnormalities of elastic and transporting properties of red blood cells under development of apoptosis |
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Authors: | I V Mindukshev V V Krivoshlyk I A Dobrylko N V Goncharov E V Vivulanets S V Kuznetsov A I Krivchenko |
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Institution: | 1.Sechenov Institute of Evolutionary Physiology and Biochemistry,Russian Academy of Sciences,St.-Petersburg,Russia;2.LUMEX Ltd,St.-Petersburg,Russia;3.Research Institute of Hygiene,Occupational Pathology and Human Ecology,St.-Petersburg,Russia;4.Pavlov Medical University,St.-Petersburg,Russia |
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Abstract: | The functional properties of erythrocytes under development of apoptotic process in these cells were investigated by the low
angle light scattering technique. Apoptosis induced by ionomycin was associated with an initial decrease of cell volume and
caused formation of echinocytes. After that the cells restored their volume forming rounded erythrocytes with rugged membrane
capable to agglomerate with each other. At the late stages of apoptosis, small fragmented cells can be revealed. Preapoptotic
red blood cells (at all stages of apoptosis) manifested an enormous tolerance to hypotonic loading, whereas control cells
hemolyzed just after reaching a critical volume (∼150 fl). Acidic hemolysis cannot differentiate between control and preapoptotic
erythrocytes, the cells being hemolyzed not reaching the critical volume. Placing the control erythrocytes to a medium with
ammonia ions instead of sodium ions caused an initial increase of cell volume above the critical point, and then it was also
followed by hemolysis. Under ammonia loading, an initial rate of the cell volume growth and a ratio of the hemolyzed cells
were significantly reduced in preapoptotic cells. |
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