A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat |
| |
| Authors: | Cristobal Uauy Francine Paraiso Pasqualina Colasuonno Robert K Tran Helen Tsai Steve Berardi Luca Comai Jorge Dubcovsky |
| |
| Affiliation: | (1) Department of Plant Sciences, University of California, Davis, CA 95616, USA;(2) Department of Genetics and Plant Breeding, University of Bari, Italy;(3) UC Davis Genome Center, University of California, Davis, CA 95616, USA;(4) John Innes Centre, Colney, Norwich, NR4 7UH, UK |
| |
| Abstract: | Background Wheat (Triticum ssp.) is an important food source for humans in many regions around the world. However, the ability to understand and modify gene function for crop improvement is hindered by the lack of available genomic resources. TILLING is a powerful reverse genetics approach that combines chemical mutagenesis with a high-throughput screen for mutations. Wheat is specially well-suited for TILLING due to the high mutation densities tolerated by polyploids, which allow for very efficient screens. Despite this, few TILLING populations are currently available. In addition, current TILLING screening protocols require high-throughput genotyping platforms, limiting their use. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
