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Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602 |
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| Authors: | Rascher Andreas Hu Zhihao Viswanathan Nina Schirmer Andreas Reid Ralph Nierman William C Lewis Matthew Hutchinson C Richard |
| Institution: | Kosan Biosciences, 3832 Bay Center Place, 94545, Hayward, CA, USA. rascher@kosan.com |
| Abstract: | We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602. |
| Keywords: | Ansamycin Antitumor Biosynthesis Cancer chemotherapy Hsp90 Polyketide synthase |
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