| 香蕉MaROP1蛋白抗体的制备 |
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| 引用本文： | 杨景豪,迟光红,徐碧玉,金志强.香蕉MaROP1蛋白抗体的制备[J].西北植物学报,2007,27(8):1583-1587. |
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| 作者姓名： | 杨景豪 迟光红 徐碧玉 金志强 |
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| 作者单位： | 中国热带农业科学院,热带生物技术研究所,海口,571101 |
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| 摘 要： | ROP(Rho-related GTPases from plants)蛋白是一类在植物信号转导方面有重要作用的蛋白质.将香蕉中的MaROP1基因连接在pET-30a载体上,并在大肠杆菌BL21(DE3)中诱导表达,获得了融合表达蛋白.将融合蛋白免疫新西兰大白兔制备了MaROP1的高效价、特异的多克隆抗体,为进一步深入研究MaROP1的生物学特性及其在香蕉果实成熟过程中的功能奠定了基础.
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| 关 键 词： | 香蕉 抗体 效价 |
| 文章编号： | 1000-4025（2007）08-1583-05 |
| 修稿时间： | 2007-04-23 |
Preparation of Banana MaROP1 Antibody |
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| YANG Jing-hao,CHI Guang-hong,XU Bi-yu,JIN Zhi-qiang.Preparation of Banana MaROP1 Antibody[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(8):1583-1587. |
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| Authors: | YANG Jing-hao CHI Guang-hong XU Bi-yu JIN Zhi-qiang |
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| Institution: | Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, H aikou 571101, China |
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| Abstract: | ROP proteins are vital regulators of signal transduction in plants and 'hot spot' in study of plant signal transduction in recent ten years.MaROP1,a cDNA encoding ROP protein,had been isolated from banana(Musa spp.) fruit.In this experiment,it was inserted in pET-30a expression vector and transformed into E.coli BL21(DE3) and the recombinant protein was obtained with IPTG inducement.The complete emulsified recombinant protein was injected into rabbit.The antiserum was verified by ELISA and Western blot.The value of antibody was very high(>10 000) and the specificity was very good(no noise stain).All of these are the groundwork of deeply researching MaROP1's function,especially for banana fruit ripening. |
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| Keywords: | MaROP1 |
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