Identification and cloning of a cyclic β-(1 → 3) β-(1 → 6)-d-glucan synthesis locus from Bradyrhizobium japonicum |
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Authors: | Arvind A. Bhagwat Raymond E. Tully Donald L. Keister |
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Affiliation: | Pathology Division, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK; Centre for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37932, USA |
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Abstract: | Abstract A gas chromatographic-mass spectrometric method was used to detect Legionella pneumophila in biofilms in potable water containing a complex microbial consortium. The unique 3-hydroxy and 2,3-dihydroxy fatty acids of the L. pneumophila lipopolysaccharides (LPS) were detected in both the planktonic phase of the continuous culture model and in the biofilms forming on both copper and polyethylene substrata. The technique confirmed that lower numbers of Legionella colonised and grew on copper in comparison to polyethylene and offers promise for routine detection of Legionella in biofilms in the environment. |
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Keywords: | Legionella Biofilms Continuous culture Lipopolysaccharide 3-Hydroxy fatty acids 2,3-Dihydroxy fatty acids |
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