广西壮族自治区HIV-1流行毒株的基因序列测定和亚型分析

使用ＰＣＲ技术对１４份广西ＨＩＶ－１阳性感染者外周血单核细胞（ＰＢＭＣｓ）样品进行扩增，获得ＨＩＶ－１膜蛋白（ｅｎｖ）基因的核酸片段，并对其Ｃ２－Ｖ３及邻区３５０－４５０个核苷酸序列进行了测定和分析。结果表明，１４份样品中９份为泰国Ｂ（Ｂ′）亚型，５份为Ｅ亚型毒株。其中Ｂ′亚型毒株的基因离散率为４．２％，与Ａ－Ｅ参考亚型及部分Ｂ亚型代表株序列相比较，与包括泰国、缅甸及云南德宏在内的Ｂ亚型毒株序列十分接近，相互之间基因离散率在３．０％－４．４％的范围内；而Ｅ亚型毒株的基因离散率为２．１％，与国际Ｅ亚型毒株的基因离散率最近，为５．６％，与其它国际参考亚型基因离散率很远，在２１．１％－２７．３％。根据以上数据及其它资料提示，广西存在Ｂ′和Ｅ两种亚型的ＨＩＶ－１的流行，且其Ｂ′亚型毒株的传入，与流行在云南德宏州的相同亚型ＨＩＶ－１毒株密切相关，而Ｅ亚型毒株则可能是由泰国经越南传入广西的

REGION OF GP120 GENES AMONG HIV-1 STRAINS IN GUANGXI AUTONOMOUS REGION OF THE ZHUANGS

HIV-1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) from 14 HIV-1 seropositive patients in Guangxi in the mid of 1996.The C2-V3 region(about 450bp) of the amplified DNA fragments were sequenced.Sequence analysis shows that there are two HIV-1 subtypes,B and E with 4.2% and 2.1% gene divergence inside each subtype.The 9 subtype B strains,after compared with sequences of several reference HIV-1 strains,were closely related to those found in Thailand,Myanmar,Yunnan and other provinces in China,and the nucleotide sequence divergence between them ranged from 3.0% to 4.4%;meanwhile,the 5 subtype E strains were most close to the international subtype E,and they are all drug users.The results above suggest that there is a recent epidemic of both B and E subtype HIV-1 strains in Guangxi.