Lowry protein determination on membrane preparations: need for standardization by amino acid analysis |
| |
Authors: | W H Peters A M Fleuren-Jakobs K M Kamps J J de Pont S L Bonting |
| |
Institution: | Department of Biochemistry, University of Nijmegen, 6500 HB Nijmegen, The Netherlands |
| |
Abstract: | The protein content of three membrane protein preparations has been determined by the Lowry method with bovine serum albumin as a standard and also by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29–42% higher values. This implies that many published data for such proteins, based on Lowry protein determinations with bovine serum albumin as the generally applied standard, are in error. Suggestions are made on how to standardize the Lowry method so that reliable values can be obtained for membrane protein. |
| |
Keywords: | To whom correspondence should be addressed |
本文献已被 ScienceDirect 等数据库收录! |