Evidence for cyanide and mercury inactivation of endogenous plastocyanin |
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| Authors: | Bruce R Selman Gary L Johnson Robert T Giaquinta Richard A Dilley |
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| Institution: | 1. Dept. of Biological Sciences, Purdue University, 47907, W. Lafayette, Indiana
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| Abstract: | Cyanide and mercury treatment of chloroplast membranes inactivates plastocyanin as shown by the inability of the extracted plastocyanin to restore electron transport in a bioassay on chloroplasts depleted of their endogenous plastocyanin by digitonin treatment. The extraction procedure did remore the enzyme from cyanide and mercury treated chloroplasts as shown by sodium dodecyl sulfate polyacrylamide electrophoresis of the extracts. This procedure normally shows a plastocyanin band at 11,000 dalton molecular weight and the band was present in extracts from control and cyanide or mercury treated membranes. |
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