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Effects of the calcium ionophore A 23187 on low-density lipoprotein processing and lipid metabolism in cultured human fibroblasts |
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| Authors: | J C Mazière C Mazière L Mora J Gardette J Polonovski |
| Institution: | Department of Biochemistry, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands |
| Abstract: | Pretreatment of cultured human fibroblasts with the calcium ionophore A 23187 resulted in a decrease in low-density lipoprotein internalization. This effect was dose-dependent and did not occur in a medium devoid of calcium. About 2-fold reduction was observed with 10(-5)M A 23187. In contrast, the low-density lipoprotein binding was only slightly affected. The incorporation of 14C]acetate and 14C]oleate into all classes of lipids (sterol, triacylglycerols and phospholipids) was strikingly reduced by ionophore pretreatment. |
| Keywords: | Electrochemistry Cyclic voltammetry Spinach ferredoxin Viologen Electrode-modification DAPV To whom correspondence should be addressed at: Department of Chemistry Georgetown University Washington DC 20057 USA |
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