Construction and regional localization of clones from a NotI linking library from human chromosome 17q |
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Authors: | J Borrow D M Black A D Goddard M K Yagle A M Frischauf E Solomon |
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Affiliation: | Somatic Cell Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom. |
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Abstract: | A NotI linking library was constructed from a somatic cell hybrid containing chromosome 17q as its only human material. A total of 112 human clones were assigned to nine regions of 17q using a somatic cell hybrid mapping panel. The library includes clones that detect the acute promyelocytic leukemia and von Recklinghausen neurofibromatosis translocation breakpoints at 17q11.2-12 and 17q11.2, respectively, on pulsed-field gel electrophoresis. The mapped clones represent over 50% of the estimated number of NotI sites on 17q, and therefore constitute an important resource for long-distance mapping. |
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