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| 几种影响籼稻成熟胚愈伤组织诱导及再生的因素 | |
| 引用本文： | 刘元风,刘彦卓,贺红,王金花,李玲,李晓方.几种影响籼稻成熟胚愈伤组织诱导及再生的因素[J].植物生理学通讯,2004,40(3):319-322. |
| 作者姓名： | 刘元风 刘彦卓 贺红 王金花 李玲 李晓方 |
| 作者单位： | 1. 广东省农业科学院水稻研究所,广州,510640;华南师范大学生命科学学院,广州,510631 2. 广东省农业科学院水稻研究所,广州,510640 3. 莱阳农学院农学系,莱阳,265200 4. 华南师范大学生命科学学院,广州,510631 |
| 摘 要： | 建立了适合5个籼稻品种成熟胚籼稻遗传转化的高效植株再生体系.N6基本培养基有利于籼稻愈伤组织的诱导和继代培养;N6大量元素和MS微量元素有利于愈伤组织的分化.降低分化培养基中蔗糖含量,加入适量山梨醇、Cu2 、Ag 和玉米素(2T)均可明显提高水稻愈伤组织的再生植株频率,5个品种分化频率均达到75%以上. |
| 关 键 词： | 籼稻 成熟胚 愈伤组织 再生植株 |
| 修稿时间： | 2003年9月15日 |
Factors Influening of Callus Induction and Plantlet Regeneration from Mature Embryos in Indica Rice |
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| LIU Yuan-Feng,LIU Yan-Zhuo,HE Hong,WANG Jin-Hua,LI Ling,LI Xiao-Fang,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou , College of Life Sciences,South ChinaNormal University,Guangzhou.Factors Influening of Callus Induction and Plantlet Regeneration from Mature Embryos in Indica Rice[J].Plant Physiology Communications,2004,40(3):319-322. | |
| Authors: | LIU Yuan-Feng LIU Yan-Zhuo HE Hong WANG Jin-Hua LI Ling LI Xiao-Fang Rice Research Institute Guangdong Academy of Agricultural Sciences Guangzhou College of Life Sciences South ChinaNormal University Guangzhou |
| Institution: | LIU Yuan-Feng1,2,LIU Yan-Zhuo1,HE Hong3,WANG Jin-Hua1,2,LI Ling2,LI Xiao-Fang1,*1Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640, 2College of Life Sciences,South ChinaNormal University,Guangzhou 510631, 3Department of Agronomy,Laiyang Agricultural College,Laiyang 265200 |
| Abstract: | A high efficient regeneration system of five indica rice suitable for genetic transformation wereestablished. N6 mediun was suitable for calli induction and subculture of indica rice. The N6 macroelementsand the MS microelements were advantageous to callus formation. By reducing sucrose and supplementingwith sorbitol, copper, silver and zeatin(ZT) to the media, the plantlet regeneration frequencies were evidentlyincreased. The plantlet regeneration frequencies of five varieties were over 75% in these media. |
| Keywords: | indica rice mature embryo callus plantlet regeneration |
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