Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid |
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Authors: | Alexandr A Prozorov Tatiana S Belova and Nikolai N Surikov |
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Institution: | (1) Institute of General Genetics, USSR Academy of Sciences, Gubkin St. 3, GSP-1, 117809 Moscow B-333, USSR |
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Abstract: | Summary During transformation of B. subtilis cells with the Bsu R restriction-modification system by means of pUB110 plasmid restriction and modification of the plasmid DNA occurs. The effect of restriction on the transformation frequency is relatively weak, bringing about 20-fold decrease only. When using cells of a modifying recipient, the frequency of AR9 phage-mediated transduction of unmodified plasmid DNA is also relatively little decreased. The frequency of transduction by chromosomal markers, under the same conditions, falls much lower. |
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