Immunofluorescence of sulphate-reducing bacteria |
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Authors: | Anthony D Smith |
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Institution: | (1) The British Petroleum Company p.l.c., Chertsey Road, TW16 7LN Sunbury-on-Thames, Middlesex, UK |
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Abstract: | An indirect fluorescent antibody technique was used as a method of rapidly assessing and identifying sulphate-reducing bacteria. Five specific antisera and one polyvalent serum were raised and tested against 44 strains of the genera Desulfovibrio and Desulfotomaculum along with 4 control organisms. Immunofluorescence was found to be mainly strain specific with the sulphate-reducing bacteria although weak fluorescence was seen both within and between recognised groups. A polyvalent antiserum was successfully used to detect sulphate-reducing bacteria. No interference from 4 control organisms was found. |
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Keywords: | Agglutination Antisera Desulfotomaculum Desulfovibrio Immunodiffusion Immunofluorescence Serology Sulphate-reducing bacteria |
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