Measurement of small scalar and dipolar couplings in purine and pyrimidine bases*期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Measurement of small scalar and dipolar couplings in purine and pyrimidine bases*

Authors:	Luká í dek, Haihong Wu, Juli Feigon Vladimí r Sklená

Affiliation:	(1) Faculty of Science, National Centre for Biomolecular Research, Masaryk University, Kotláská 2, 61137 Brno, Czech Republic;(2) Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California, 90024, U.S.A

Abstract:	A suite of spin-state-selective excitation (S³E) NMR experiments for the measurements of small one-bond (¹³C-¹³C, ¹⁵N-¹³C) and two-bond (¹H-¹³C, ¹H-¹⁵N) coupling constants in ¹³C,¹⁵N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between and sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S³E rather than /-half-filter are demonstrated using results obtained on isotopically labeled DNA oligonucleotides.

Keywords:	NMR methods nucleic acid bases residual dipolar couplings spin-state-selective filters
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