Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene |
| |
| Authors: | K Rajasekaran J W Cary TJ Jacks K D Stromberg TE Cleveland |
| |
| Institution: | (1) USDA, ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA Fax: +1–504–2864217 e-mail: krajah@nola.srrc.usda.gov, US;(2) Department of Biology, University of Southwestern Louisiana, Lafayette, LA 70504, USA, US |
| |
| Abstract: | Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays
of CPO-P enzyme activity. Extracts from plants transformed with the CPO-P gene significantly reduced Aspergillus flavus colonies by up to 100% compared with extracts from control plants transformed with pBI121. Compared with controls, the transformed
plants showed increased disease resistance in planta against a fungal pathogen, Colletotrichum destructivum, the causal agent of tobacco anthracnose.
Received: 10 March 1999 / Revision received: 22 June 1999 · Accepted: 5 July 1999 |
| |
| Keywords: | Chloroperoxidase Disease resistance Haloperoxidase Reactive oxygen species Tobacco |
| 本文献已被 SpringerLink 等数据库收录！ |
|
