A new interferometric technique for the determination of the electrophoretic mobility of macromolecules. |
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| Authors: | W F Pickard K C Sehgal |
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| Institution: | 1. Department of Electrical Engineering, Washington University, Saint Louis, Missouri 63130 USA;2. Department of Biological Chemistry, Washington University, Saint Louis, Missouri 63130 USA |
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| Abstract: | A novel device is described for measuring the electrophoretic mobility of monodisperse macromolecules. This apparatus, which is based upon a Twyman-Green interferometer, yields ±3% precision with runs lasting only 15 min; and it is suitable for temperatures and/or ionic strengths near or below physiological. Its operation is illustrated with bovine serum albumin, the apparent mobility of which is shown to vary strongly with the purity and concentration of the preparation. |
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