Immunohistochemical localization of beta-LPH in the human hypothalamus. |
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Authors: | G Pelletier L Désy J C Lissitszky F Labrie C H Li |
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Institution: | 1. Medical Research Council Group in Molecular Endocrinology, Le Centre Hospitalier de l''Université Laval, Quebec G1V4G2, Canada;2. Hormone Research Laboratory, University of California, San Francisco, Calif. 94143, USA |
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Abstract: | In order to establish the presence of β-LPH and to clearly identify the nervous structures containing β-LPH in the human hypothalamus, an immunohistochemical localization of β-LPH was performed in this tissue. The immunohistochemical technique involved use of a specific antiserum to human β-LPH and the peroxidase-antiperoxidase complex. Immunostained neuronal cell bodies were observed in the arcuate nucleus whereas β-LPH-positive nervous fibers could be detected in a large area extending rostro-caudally from the anterior part of the paraventricular nucleus up to the mammillary bodies. Staining was completely abolished by previous immunoabsorption with β-LPH while β-endorphin and ovine γ-LPH1–47 only partially prevented immunostaining. Although it cannot be excluded that the precursor 31K molecule, β-LPH1–58 and/or β-endorphin are detected by the immunostaining, it is likely that β-LPH is at least partly responsible for the positive reaction. |
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