Laser Raman studies of the 5 S rRNA-protein L5 complex of rat liver ribosomes |
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Authors: | Heinz Fabian Siegfried Böhm Wolfgang Carius Rolf Misselwitz Heinz Welfle |
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Affiliation: | 1. Central Institute of Molecular Biology, GDR Academy of Sciences, DDR-1115 Berlin-Buch, GDR;2. Pedagogic University ‘Dr. Theodor Neubauer’, DDR-5000 Erfurt, GDR |
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Abstract: | The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions appear to be present in the complex with protein L5 as well as in the free 5 S rRNA. Furthermore specific interactions between 5 S rRNA and protein L5 are indicated. Cytosine (and/or uracil) residues in single-stranded regions and the N(7) of guanine are engaged in interactions with the protein as suggested by the Raman data. |
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Keywords: | 5 S rRNA Ribosomal protein L5 RNA—protein complex Raman spectroscopy Rat liver |
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