Recombinant Hansenula polymorpha as a biocatalyst: coexpression of the spinach glycolate oxidase (GO) and the S. cerevisiae catalase T (CTT1) gene |
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Authors: | G Gellissen M Piontek U Dahlems V Jenzelewski J E Gavagan R DiCosimo D L Anton Z A Janowicz |
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Institution: | (1) Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Düsseldorf, Germany, DE;(2) DuPont Central Science & Engineering, Experimental Station, Wilmington, Delaware 19880-0328, USA, US |
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Abstract: | The methylotrophic yeast Hansenula polymorpha has been developed as an efficient production system for heterologous proteins. The system offers the possibility to cointegrate
heterologous genes in anticipated fixed copy numbers into the chromosome. As a consequence coproduction of different proteins
in stoichiometric ratios can be envisaged. This provides options to design this yeast as an industrial biocatalyst in procedures
where several enzymes are required for the efficient conversion of a given inexpensive compound into a valuable product. To
this end recombinant strains have been engineered with multiple copies of expression cassettes containing the glycolate oxidase
(GO) gene from spinach and the catalase T (CTT1) gene from S. cerevisiae. The newly created strains produce high levels of the peroxisomal glycolate oxidase and the cytosolic catalase T. The strains
efficiently convert glycolate into glyoxylic acid, oxidizing the added substrate and decomposing the peroxide formed during
this reaction into water and oxygen.
Received: 31 October 1995/Received last revision: 23 February 1996/Accepted: 4 March 1996 |
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