An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment |
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| Authors: | Harvey Jeffrey Bergdahl Andreas Dadafarin Hesam Ling Li Davis Elaine C Omanovic Sasha |
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| Institution: | (1) Department of Chemical Engineering, McGill University, University Street, Montreal, QC, 3610, Canada;(2) Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada;(3) Present address: Department of Exercise Science, Concordia University, Montreal, QC, Canada; |
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| Abstract: | An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface
was developed. The method was efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic
acid) surface layer. This layer was used as a ‘linker’ to immobilize the extracellular matrix protein fibronectin to the 316L
stainless steel surface. Fibronectin was irreversibly attached to the surface and, unlike physisorbed fibronectin, resisted
detachment more in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was more
biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25%
improvement in cell density. |
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