Plant regeneration from nucllar tissues of Aegle marmelos through organogenesis |
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Authors: | M. Hossain M. R. Karim R. Islam O. I. Joarder |
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Affiliation: | (1) Department of Botany, University of Rajshahi, 6205 Rajshahi, Bangladesh |
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Abstract: | A protocol for organogenesis from nucellar explants excised from fertilized ovules of immature fruits of Aegle marmelos Corr. was developed. Adventitious buds were initiated on Murashige and Skoog's (MS) medium containing various combinations of 6-benzyladenine (BA), -naphthalene-acetic acid (NAA), 3-indoleacetic acid and gibberellic acid. Medium containing 4.4 m BA and 2.7 M NAA produced the maximum number of adventitious buds per explant. Shoots were elongated by transferring explants with shoot buds to medium with a low concentration of BA (0.44 M). Rooting of in vitro-regenerated shoots was obtained in half-strength MS medium with 4.9 M indole-3-butyric acid. This is the first report of plant regeneration from nucellar explants of A. marmelos.Abbreviations BA benzyladenine - IAA indoleacetic acid - IBA indolebutyric acid - NAA naphthaleneacetic acid - GA3 gibberellic acid |
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Keywords: | adventitious bud induction nueellus eulture |
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