ET抗体对葡萄球菌性烫伤样皮肤综合征的免疫保护作用

目的探讨ET抗体对葡萄球菌性烫伤样皮肤综合征的免疫保护作用。方法用重组ETA和ETB建立检测血清ETA和ETB抗体的间接ELISA法，用建立的ELISA法测定商业化人高效价丙种球蛋白（IVlg）、葡萄球菌性烫伤样皮肤综合征（SSSS）患儿、特应性皮炎（AD）患儿和健康儿童血清中ETA和ETB抗体的效价。用重组ETA和ETB皮下注射新生小鼠建立SSSS动物模型，在注射重组ETA和ETB的同时和3h后在小鼠腹腔内注射ETA和ETB抗体，观察小鼠发病情况和皮肤组织病理改变。结果IVlg中测出高效价的ETA和ETB抗体；SSSS患儿、AD患儿和健康儿童血清中ETB抗体效价（A450nm）分别为0．863±0．276、1．027±0．222、0．990±0．151。SSSS患儿血清中ETB抗体效价明显低于AD患儿和健康儿童，差异有统计学意义（P〈0．05），但三者问ETA抗体的效价差异无统计学意义（P〉0．05）。注射ETA和ETB抗体的新生小鼠发病、皮肤组织病理改变和死亡率均低于仅注射重组ETA和ETB小鼠。结论ET抗体对葡萄球菌性烫伤样皮肤综合征具有免疫保护作用，以ETB抗体为主，可减轻皮肤损伤，降低死亡率。

Protective role of ET antibody against staphylococcal scalded skin syndrome

Objective To explore the protective role of ET antibody in staphylococcal scalded skin syndrome （SSSS）. Methods Recombinant ETA and ETB antigens were used to establish an indirect ELISA method for the detection of the antibodies of ETA and ETB in serum. The titers of ETA and ETB antibodies in the commercial high titer human J3 Ig （IVIg） , the sera of children with SSSS/atopic dermatitis （AD） , and in the sera of healthy chil- dren were detected by the above ELISA. The mouse model of SSSS was produced by injecting subcutaneously re- combinant ETA and ETB at the nape of the neck of newborn mice. The antibodies of ETA and ETB were injected intraperitoneally at the same time of and 3 hours after the injection of recombinant ETA and ETB. The onset of dis- ease and the pathologic changes in skin tissue were observed. Results The high titers of antibodies of ETA and ETB were detected in IVIg. The titers of ETB antibody （A450 nm） were 0. 863 ± 0. 276, 1. 027 ± 0. 222, and0. 990 ±0. 151 respectively in the sera of the SSSS, AD, and normal groups. The titers of ETB antibody of the children with SSSS were lower obviously than those of the children with AD and of the normal children, and the difference was significant （ P 〈 0.05）. There were no significant differences （ P 〈 0.05 ） in the titers of ETA anti- body among the three groups （P 〉 0.05 ）. The onset of disease, pathologic changes in skin tissue and the death rate of the mice injected the antibodies of ETA and ETB were superior to those of the mice injected only recombi- nant ETA and ETB. Conclusion ET antibodies, especially the ETB antibodies, have a protective role against staphylococcal scalded skin syndrome. They can alleviate the damage to skin and reduce the death rate.