Phylogenetic study of sponge associated bacteria from the Lakshadweep archipelago and the antimicrobial activities of their secondary metabolites |
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Authors: | M Gopi T T Ajith Kumar R Balagurunathan R Vinoth K V Dhaneesh R Rajasekaran T Balasubramanian |
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Institution: | (1) CMLRE Field Research Station, Agatti Island, Lakshadweep, 682553, India;(2) CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608502, India;(3) Department of Microbiology, Periyar University, Salem, 636011, India |
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Abstract: | Marine ecosystem of the Lakshadweep archipelago is unique and known to have a very high degree of biodiversity with a number
of endemic flora and fauna. The present study focuses to isolate the endosymbiotic microorganism from sponges and its effectiveness
against marine ornamental fish pathogens. The sponges were collected from Agatti island of Lakshadweep archipelago and identified
as Clathria procera, Sigmadocia fibulata and Dysidea granulosa. In which, 15 different types of bacteria were isolated and screened against marine ornamental fish pathogens (A. hydrophila, Vibrio alginolyticus, V. harveyii, V. parahaemolyticus and Pseudomonas fluorescens). The strain S25 was found as potential bacteria based on their antimicrobial activity against the fish pathogens. Molecular
identification of the potential strain (S25) of the 16S rRNA gene showed 99% identity with Acinetobacter sp. The sequenced 16 s rRNA gene with 1,081 bp in length was submitted in NCBI Genbank and Accession was obtained (GenBank
Accession number HM004071). The strain exhibited high similarity (99%) with the 16S rRNA gene of Acinetobacter calcoaceticus from GenBank database. Crude extract obtained with acetone and ethyl acetate from extracellular products of S25 showed significant
antimicrobial activity by disc diffusion assay using 1,500 μg/ml of crude extract. Extracellular metobolite of A. calcoaceticus was extracted by shake flask method and the crude extract was partially purified by thin layer chromatography. Partially
purified crude extract showed significant inhibition zone of antimicrobial activity (A. hydrophila, V. alginolyticus, V. parahaemolyticus) and less similar activity against V. harveyii and P. fluorescens. This is the first report on A. calcoaceticus isolated from sponges of Lakshadweep archipelago and the studies are underway to characterize and purify the antimicrobial
compounds of the potential bacteria. |
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