Identification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis |
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Authors: | Jungha Park Jun Kawamoto Nobuyoshi Esaki Tatsuo Kurihara |
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Institution: | (1) Institute for Chemical Research, Kyoto University, Uji Kyoto, 611-0011, Japan; |
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Abstract: | Shewanella livingstonensis Ac10 is a psychrotrophic Gram-negative bacterium that grows at temperatures close to 0°C. Previous proteomic studies of this
bacterium identified cold-inducible soluble proteins and outer membrane proteins that could possibly be involved in its cold
adaptation (Kawamoto et al. in Extremophiles 11:819–826, 2007). In this study, we established a method for separating the inner and outer membranes by sucrose density gradient ultracentrifugation
and performed proteomic studies of the inner membrane fraction. The cells were grown at temperatures of 4 and 18°C, and phospholipid-enriched
inner membrane fractions were obtained. Two-dimensional polyacrylamide gel electrophoresis and peptide mass fingerprinting
analysis of the proteins identified 14 cold-inducible proteins (more than a 2-fold increase at 4°C). Six of these proteins
were predicted to be inner membrane proteins. Two predicted periplasmic proteins, 5 predicted cytoplasmic proteins, and 1
predicted outer membrane protein were also found in the inner membrane fraction, suggesting their association with the inner
membrane proteins and/or lipids. These cold-inducible proteins included proteins that are presumed to be involved in chemotaxis
(AtoS and PspA), membrane protein biogenesis (DegP, SurA, and FtsY), and morphogenesis (MreB). These findings provide a basis
for further studies on the cold-adaptation mechanism of this bacterium. |
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