Increased accumulation of anthocyanins in transgenic potato tubers by overexpressing the 3GT gene |
| |
Authors: | Qing Wei Quan-Yi Wang Zhi-Hang Feng Bing Wang Yun-Feng Zhang Qing Yang |
| |
Affiliation: | (1) College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China;(2) Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, 223300 Huaian, China; |
| |
Abstract: | In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar Désirée plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|