Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene |
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Authors: | Park Hyang-Mi Choi Man-Soo Kwak Do-Yeon Lee Bong-Choon Lee Jong-Hee Kim Myeong-Ki Kim Yeon-Gyu Shin Dong-Bum Park Soon-Ki Kim Yul-Ho |
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Institution: | (1) State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, 271018 Tai’an, People’s Republic of China;(2) Rice Science Research Institute, Shandong Academy of Agricultural Science, 272017 Jining, People’s Republic of China; |
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Abstract: | Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly
RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH),
and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed
by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance
trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants. |
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Keywords: | movement protein (MP) Oryza sativa L rice stripe virus (RSV) RNAi RSV coat protein silencing suppressor (NS3) small brown planthopper (SBPH) |
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