Calcitonin mRNA activity in young obese (fa/fa) Zucker rats |
| |
Authors: | N Segond A Jullienne E H Tahri J M Garel |
| |
Institution: | 1. Unité 113 de L''INSERM, CHU Saint-Antoine, 27 Rue Chaligny, 75571 Paris Cédex 12 France;2. GroupeHormones du Calcium et Développement, Université Pierre et Marie Curie, 4 Place Jussieu, 75230 Parix Cédex 05, France |
| |
Abstract: | Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in obese animals, but plasma phosphate was unchanged. Plasma CT levels measured by radioimmunoassay (RIA) were significantly decreased in fatty (0.50 +/- 0.03 vs 0.68 +/- 0.03 ng/ml in Leans; P less than 0.001), but thyroidal hormone content was not different between Lean and fatty rats (68.7 +/- 5.1 in Leans vs 60.5 +/- 3.6 ng/gland in fatty rats). mRNA was extracted from 10 thyroids, and translated in a rabbit reticulocyte lysate (NEN) in the presence of 35S]methionine. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographed and quantified by integration. A 50% decrease in translatable CT mRNA was observed in fatty rats. In basal conditions, the biosynthetic activity of C cells in obese rats correlates with the secretion rate of the hormone in the face of unchanged thyroidal CT contents. |
| |
Keywords: | mRNA activity Calcitonin Young obese rat Zucker rat |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|