Effect of insulin on glucagon binding to isolated rat epididymal adipocytes |
| |
| Authors: | K Hashizume K Yamauchi |
| |
| Affiliation: | 1. Univ. Lille, EA 7394– ICV – Institut Charles Viollette, F-59000 Lille, France;2. CUMA, Faculté de Pharmacie, Lille, France;3. Univ. Lille, EA 4489, Unité Environnement Périnatal et Croissance - Équipe Dénutritions Maternelles Périnatales, F-59000 Lille, France;1. Department of Psychology, Faculty of Human Sciences, Medical School Hamburg, Hamburg, Germany;2. ICPP Institute of Clinical Psychology and Psychotherapy, Medical School Hamburg, Hamburg, Germany;3. ICAN Institute for Cognitive and Affective Neuroscience, Medical School Hamburg, Hamburg, Germany |
| |
| Abstract: | Effect of insulin on glucagon binding to rat epididymal adipocytes was studied in vitro. [125I]iodoglucagon binding to isolated adipocytes was increased by preincubation of the cells with insulin. Maximal increase was observed with 7 X 10(-10) M insulin. In Scatchard analysis, [125I]iodoglucagon competition data generated one binding site with a single affinity for glucagon binding in the cells pretreated with buffer alone. Pretreatment of the cells with insulin increased the affinity without changes in the number of binding sites. [125I]iodoglucagon binding to isolated adipocytes was not affected by pretreatment of the cells with luteinizing hormone, follicle-stimulating hormone, growth hormone, or with prolactin. These results suggest that insulin stimulates glucagon binding to adipocytes. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
