Different roles of galectin-9 isoforms in modulating E-selectin expression and adhesion function in LoVo colon carcinoma cells |
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Authors: | Feng Zhang Minhua Zheng Ying Qu Jianfang Li Jun Ji Bo Feng Aiguo Lu Jianwen Li Mingliang Wang Bingya Liu |
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Affiliation: | (1) Department of General Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025, China;(2) Department of General Surgery, The Third Hospital, Soochow University, Changzhou, 213003, China |
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Abstract: | Galectin-9, a member of galectin family, plays multiple roles in a variety of cellular functions, including cell adhesion, aggregation, and apoptosis. Galectin-9 also has three isoforms (named galectin-9L, galectin-9M, and galectin-9S), but whether these isoforms differ in their functions remains poorly understood. In this study, we showed that transient expression of galectin-9L decreased E-selectin levels, while transient expression of galectin-9M or galectin-9S increased E-selectin levels in LoVo cells, which do not express endogenous galectin-9. We also found that over-expression of three galectin-9 isoforms led to increased attachment of LoVo cells to extracellular matrix proteins respectively, while over-expression of galectin-9M or galectin-9S increased the adhesion of LoVo cells to human umbilical vein endothelial cells in vitro. In summary, these findings indicate that different isoforms of galectin-9 exhibit distinct biological functions. |
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Keywords: | KeywordHeading" >: Galectin-9 isoforms E-selectin Adhesion Colon carcinoma |
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