Genetically engineered human interleukin-6 variant with enhanced stability |
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| Authors: | S.C. Lee M.J. Lee I. Choi M.-H. Yu |
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| Affiliation: | (1) Protein Folding Research Unit, Korea;(2) Immune Cell Signal Transduction Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong Taejon, 305-600, Korea |
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| Abstract: | A recombinant human interleukin-6 mutant with enhanced conformational stability toward denaturant was obtained by site-specific mutagenesis. The clone was identified as having a single amino acid substitution of Lys70Glu. When urea-induced denaturation was monitored by the change in fluorescence intensity at 360 nm, Lys70Glu mutation shifted the midpoint of unfolding transition from 5.8 M (wild type) to 6.6 M urea. This mutation did not impair the biological activity. |
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