Recovery of ranavirus dsDNA from formalin-fixed archival material |
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Authors: | Kattenbelt J A Hyatt A D Gould A R |
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Institution: | CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia. |
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Abstract: | The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the examination of ranavirus DNA within archival tissues thereby providing valuable data for identifying the origin and tracing the spread of ranaviruses. |
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