Structure of the intergenic spacer region from the ribosomal RNA gene family of white spruce (Picea glauca) |
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Authors: | Robin Nicholas Beech Curtis Strobeck |
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Affiliation: | (1) Department of Zoology, Biological Sciences Centre, University of Alberta, T6G 2E9 Edmonton, Alberta, Canada;(2) Present address: Institute of Parasitology, MacDonald College, McGill University, 21, 111 Lakeshore Road, H9X 3V9 Ste Anne de Bellevue, Quebec, Canada |
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Abstract: | Five genomic clones containing ribosomal DNA repeats from the gymnosperm white spruce (Picea glauca) have been isolated and characterized by restriction enzyme analysis. No nucleotide variation or length variation was detected within the region encoding the ribosomal RNAs. Four clones which contained the intergenic spacer (IGS) region from different rDNA repeats were further characterized to reveal the sub-repeat structure within the IGS. The sub-repeats were unusually long, ranging from 540 to 990 bp but in all other respects the structure of the IGS was very similar to the organization of the IGS from wheat, Drosophila and Xenopus. |
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Keywords: | intergenic spacer length variation Picea glauca ribosomal DNA white spruce |
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