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Molecular cloning of AKR xenotropic murine leukemia virus unintegrated proviral DNA |
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| Authors: | D R Joseph |
| Affiliation: | Robert V. Slater Laboratory, Papanicolaou Cancer Research Institute, 1155 N.W. 14th Street, Miami, FL 33136 U.S.A. |
| Abstract: | Suprahelical proviral DNA of AKR xenotropic murine leukemia virus was purified from agarose gels and cloned in lambda Charon 28 DNA (BamHI sites). Nine viral DNA recombinants were identified and mapped with 12 restriction endonucleases. Three calsses of cloned viral DNA inserts were found: (1) Six inserts were apparently full-length 9.0-kb DNA with tandem long terminal repeat (LTR) elements; (2) two inserts contained DNAs with deletions in or adjacent to the LTR regions; (3) a single isolate contained an inversion of 2.3 kb around the LTR in the envelope gene. |
| Keywords: | Recombinant DNA Charon 28 lambda vector restriction endonuclease mapping deleted and inverted forms cDNA DNA complementary to viral RNA kb kilobase pairs LTR long terminal repeat MuLV murine leukemia virus MuX xenotropic MuLV (not infectious to mouse cells but infectious to some heterologous cells) |
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