Membrane and cytoplasmic resistivity properties of normal and sickle red blood cells |
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Authors: | Gary V. Richieri Howard C. Mel |
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Affiliation: | (1) Department of Biophysics and Medical Physics, University of California, Berkeley, 94720 Berkeley, CA |
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Abstract: | The cytoplasmic resistivities and membrane breakdown potentials of normal (AA), sickle-cell-trait (AS), and sickle (SS) red blood cells have been measured by the biophysical methodology of resistive pulse spectroscopy over a range of osmolalities. At isotonicity, the average membrane breakdown potentials are virtually identical for the three types of cells occurring at about 1150 V/cm. Average isotonic cytoplasmic resistivities are somewhat higher for the SS cells (166.7±7.49 ohm-cm) compared to the AA (147.6±1.98 ohm-cm) or AS cells (148.7±1.79 ohm-cm). As medium osmolality is varied, the differences in resistive properties become enlarged, especially at very low and very high osmolalities. At high osmolalities, both types of sickle cells show a large increase in internal resistivity compared to the normals; at low osmolality, the SS samples exhibit a distinctly different membrane breakdown characteristic, decreasing in this parameter, whereas the other two groups increase. Of the 15 SS samples tested, three displayed much higher cytoplasmic resistivities at isotonicity: 218.2±5.25 ohm-cm, compared to an average of 153.5±3.46 ohm-cm for the other 12. The relationship between these high resistivities and the subfraction of irreversibly sickled cells in the sample is discussed. |
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Keywords: | Sickle red blood cells, membrane and ctytoplasmic resistivity properties of red blood cells, membrane and cytoplasmic resistivity properties of membrane breakdown properties cytoplasmic resistivity electronic particle sizing osmotic responses sickle cells sickle cell trait cell shape factors |
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