Electron microscopic observations of synaptic endings in cultures of mammalian central nervous tissue

Summary Synapses were found in rat cerebellar and brainstem cultures with the electron microscope. Three distinct types of synaptic terminals were described. The similarity between synapses found in vitro and in vivo was emphasized.Supported by USPHS Grants 5 Tl 459-04 and NB 03114-03S1 from the National Institutes of Health, Bethesda, Maryland.The authors wish to express their sincere appreciation to Mrs. Eleanor Morris for her assistance in preparing the cultures and Mr. Earl Pitsinger for his photographic assistance.