生长调节剂对黄连木光合生理指标和荧光参数的影响

以黄连木幼苗为试材,采用叶片喷施的方法,研究了生长调节剂多效唑和矮壮素对黄连木叶片解剖结构、色素含量、光合生理指标和叶绿素荧光参数的影响,以揭示生长调节剂对黄连木光合特性的影响及其内在机制。结果显示:随着两种生长调节剂处理浓度的增加,黄连木幼苗叶片厚度、栅栏组织厚度、海绵组织厚度、色素含量、净光合速率(Pn)、PSⅡ原初光能转化效率(Fv/Fm)、PSⅡ的潜在活性(Fv/F0)、叶片单位面积吸收的能量(ABS/CS)和捕获的能量(TRo/CS)以及电子传递的能量(ETo/CS)均呈先增大后减小的趋势,并均在800mg/L多效唑和500mg/L矮壮素处理时达到最大值,且显著提高了黄连木叶片的净光合速率。研究发现,黄连木幼苗叶片栅/海比例增大和叶绿素含量的增加使叶片对光能的捕获和吸收能力提高,而PSⅡ反应中心开放比例的增大与其活性的增强保证了叶片对光能的高效传递,同时降低了能量的热耗散,是提高苗木光合速率和保证苗木光合作用高效进行的内在机制。

Effects of Growth Regulators on Photosynthetic and Physiological Indices and Chlorophyll Fluorescence Parameters of Pistacia chinensis

With Pistacia chinensis as materials,which were treated by foliar spray method,this paper studied that the effects of PP333 and CCC treatment on leaf anatomical structure,pigment content,photosynthetic and physiological indices and chlorophyll fluorescence parameters.The results were as follows:the leaf thickness,palisade tissue thickness,sponge tissue thickness and pigment content firstly increased and then decreased with increasing concentrations of growth regulators,as the same as the net photosynthetic rate(Pn),ratio of variable to maximum fluorescence(Fv/Fm),potential photochemical efficiency(Fv/F0),energy fluxes per excited cross-sections for absorption(ABS/CS),trapping(TRo/CS) and electron transport(ETo/CS).All of these indices reached the maximum when PP333 concentration is 800 mg/L and CCC concentration is 500 mg/L,and the results indicated that the net photosynthetic rate(Pn) of P.chinensis was significantly increased with 800 mg/L of PP333 and 500 mg/L of CCC.Study found that the increase of thickness ratio of palisade tissue and sponge tissue and content of chlorophyll improved the ability of light capture and absorption light energy of leaves,and the increase of openness degree and actinity of PSⅡ reaction centers ensured the efficient transfer of light energy and reduced the heat dissipation of energy.All of above is the response mechanism that growth regulators increase the net photosynthetic rate of nursery stock and ensure the efficient photosynthesis of nursery stock.