Various means of integration of the expressible human dihydrofolate reductase gene into the Bacillus subtilis genome |
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| Authors: | A A Prozorov E U Poluektova G V Savchenko V Z Nezametdinova F K Khasanov |
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| Affiliation: | Laboratory of Genetics of Microorganisms, N.I. Vavilov Institute of General Genetics, U.S.S.R. Academy of Sciences, Moscow. |
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| Abstract: | Integration of expressible DNA corresponding to the human dihydrofolate reductase (hDHFR) gene into the Bacillus subtilis genome has been achieved in different ways. The clones obtained contained one to seven copies of this gene per genome equivalent and were resistant to trimethoprim. Clones produce a new protein coded by the integrated hDHFR gene. In all clones, the integrated DNA was stably maintained even under nonselective growth conditions. |
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