Response of methanotrophs and methane oxidation on ammonium application in landfill soils |
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Authors: | Na Yang Fan Lü Pinjing He Liming Shao |
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Institution: | (1) State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, People’s Republic of China; |
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Abstract: | To test the dose effect of ammonium (NH4
+) fertilization on soil methane (CH4) oxidation by methanotrophic communities, batch incubations were conducted at a wide scale of NH4
+ amendments: 0, 100, 250, 500, and 1,000 mg N kgdry soil
−1. Denaturing gradient gel electrophoresis and real-time quantitative PCR analysis were conducted to investigate the correlation
between the CH4 oxidation capacity and methanotrophic communities. Immediately after the addition of NH4
+, temporal inhibition of CH4 oxidation occurred, and this might have been due to the non-specific salt effect (osmotic stress). After a lag phase, the
CH4 oxidation rates of the soils with NH4
+ fertilization were promoted to levels higher than those of the controls. More than 100 mg N kgdry soil
−1 of NH4
+ addition resulted in the reduction of type II/type I MOB ratios and an obvious evolution of type II MOB communities, while
less than 100 mg N kgdry soil
−1 of NH4
+ addition induced nearly no change of methanotrophic community compositions. The NH4
+-derived stimulation after the lag phase was attributed to the improvement of N availability for type I MOB. Compared with
the controls, 100 mg N kgdry soil
−1 of NH4
+ addition doubled the CH4 oxidation peak value to more than 20 mg CH4 kgdry soil
−1 h−1. Therefore, an appropriate amount of leachate irrigation on the landfill cover layer might efficiently mitigate the CH4 emissions. |
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Keywords: | |
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