|
|||||
|
|
Three exopolysaccharides of the beta-(1-->6)-D-glucan type and a beta-(1-->3;1-->6)-D-glucan produced by strains of Botryosphaeria rhodina isolated from rotting tropical fruit |
|
| Authors: | Vasconcelos Ana Flora D Monteiro Nilson K Dekker Robert F H Barbosa Aneli M Carbonero Elaine R Silveira Joana L M Sassaki Guilherme L da Silva Roberto de Lourdes Corradi da Silva Maria |
| Institution: | a Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CP 467, CEP 19060-900, Presidente Prudente, São Paulo, Brazil b Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista, CP 199, CEP 13506-900, Rio Claro, São Paulo, Brazil c Universidad de Castilla—La Mancha, Instituto de Regional Investigación Científica Aplicada (IRICA), 13071 Ciudad Real, Spain d Departamento de Bioquímica e Biotecnologia—CCE, Universidade Estadual de Londrina, CP 6001, CEP 86051-990, Londrina, Paraná, Brazil e Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, Paraná, Brazil f Departamento de Química e Ciências Ambientais, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, CEP 15054-000, São José do Rio Preto, São Paulo, Brazil |
| Abstract: | Four exopolysaccharides (EPS) obtained from Botryosphaeria rhodina strains isolated from rotting tropical fruit (graviola, mango, pinha, and orange) grown on sucrose were purified on Sepharose CL-4B. Total acid hydrolysis of each EPS yielded only glucose. Data from methylation analysis and (13)C NMR spectroscopy indicated that the EPS from the graviola isolate consisted of a main chain of glucopyranosyl (1-->3) linkages substituted at O-6 as shown in the putative structure below: carbohydrate structure: see text]. The EPS of the other fungal isolates consisted of a linear chain of (1-->6)-linked glucopyranosyl residues of the following structure: carbohydrate structure: see text]. FTIR spectra showed one band at 891 cm(-1), and (13)C NMR spectroscopy showed that all glucosidic linkages were of the beta-configuration. Dye-inclusion studies with Congo Red indicated that each EPS existed in a triple-helix conformational state. beta-(1-->6)-d-Glucans produced as exocellular polysaccharides by fungi are uncommon. |
| Keywords: | Botryosphaeria rhodina isolates Exopolysaccharides β (1→ d-Glucans" target="_blank">6)-d-Glucans β (1→ 3 1→ d-Glucans" target="_blank">6)-d-Glucans Triple-helix conformation |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |