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| 应用16S rDNA克隆文库解析人工快速渗滤系统细菌种群多样性 | |
| 引用本文： | 马鸣超,姜 昕,李 俊,王 静. 应用16S rDNA克隆文库解析人工快速渗滤系统细菌种群多样性[J]. 微生物学通报, 2008, 35(5): 0731-0736 |
| 作者姓名： | 马鸣超 姜 昕 李 俊 王 静 |
| 作者单位： | 1. 中国地质大学,水资源与环境学院,水资源与环境工程北京市重点实验室,北京,100083 2. 中国地质大学,水资源与环境学院,水资源与环境工程北京市重点实验室,北京,100083;中国农业科学院农业资源与农业区划研究所,北京,100081 3. 中国农业科学院农业资源与农业区划研究所,北京,100081 |
| 摘 要： | 本文出不穷通过构建16S rDNA克隆文库开展了人工快速渗滤(CRI)系统表层(10cm深)细菌种群多样性研究,结果表明,在CRI系统表层填料中细菌多样性十分丰富,存在7个主要类群,其中不可培养的酸杆菌纲(uncultured Acidobacteria)和其它不可培养细菌(uncultured bacteria)在整个克隆文库中比例最大,比例为53.72%,其次是浮霉菌纲(uncultured planctomycete)和β-变形菌纲(β-proteobacterium),分别占文库比例的13.89%和8.33%;克隆文库中反硝化菌的比例高于亚硝化单胞菌属细菌(O.925%),没有出现Nitrospira属的克隆子.本文通过16S rDNA克隆文库揭示了在生物膜上存在的优势茵为不可培养菌,而假单胞菌(Pseudomonas aeruginosa)和紫色色杆菌(Chromobacterium sp.)等在平板分离培养中出现的频率较高,两种方法之间的结果存在差异.本研究采用16S rDNA克隆文库方法揭示的结果,将为CRI系统生物降解的进一步提高提供依据. |
| 关 键 词： | 人工快速渗滤系统(CRI) 16S rDNA克隆文库 细菌种群多样性 应用 rDNA 克隆文库 快速 渗滤系统 细菌 种群多样性 System Infiltration Rapid Sampling Clone Library Community Composition Bacterial 生物降解 研究 差异 方法 频率 分离培养 |
Analysis of Bacterial Community Composition by 16S rDNA Clone Library Sampling from Constructed Rapid Infiltration System(CRI) |
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| MA Ming-Chao,JIANG Xin,LI Jun and WANG Jing. Analysis of Bacterial Community Composition by 16S rDNA Clone Library Sampling from Constructed Rapid Infiltration System(CRI)[J]. Microbiology China, 2008, 35(5): 0731-0736 | |
| Authors: | MA Ming-Chao JIANG Xin LI Jun WANG Jing |
| Affiliation: | 1. Beijing Key Laboratory of Water Resources & Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing 100083;(1. Beijing Key Laboratory of Water Resources & Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing 100083)(2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricul;2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Science, Beijing 100081;1. Beijing Key Laboratory of Water Resources & Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing 100083 |
| Abstract: | |
| Keywords: | Constructed Rapid Infiltration system (CRI) 16S rDNA clone library Bacterial community composition |
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