Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge |
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Authors: | Kotay Shireen Meher Das Debabrata |
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Affiliation: | Fermentation Technology Laboratory, Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India. |
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Abstract: | Bacillus coagulans strain IIT-BT S1 isolated from anaerobically digested activated sewage sludge was investigated for its ability to produce H(2) from glucose-based medium under the influence of different environmental parameters. At mid-exponential phase of cell growth, H(2) production initiated and reached maximum production rate in the stationary phase. The maximal H(2) yield (2.28 mol H(2)/molglucose) was recorded at an initial glucose concentration of 2% (w/v), pH 6.5, temperature 37 degrees C, inoculum volume of 10% (v/v) and inoculum age of 14 h. Cell growth rate and rate of hydrogen production decreased when glucose concentration was elevated above 2% w/v, indicating substrate inhibition. The ability of the organism to utilize various carbon sources for H(2) fermentation was also determined. |
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