Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase |
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| Authors: | M M Van Lookeren Campagne J Franke R H Kessin |
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| Institution: | Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York 10032. |
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| Abstract: | A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with a predicted molecular mass of 79.6 kDa. The Dictyostelium enzyme has extensive homology with the GMP synthetase of Escherichia coli and regional homology to other glutamine amidotransferases. |
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